Biochemistry and Systems Biology Seminar - Dr. Yijun Ruan (Beatty Lecture)
Dr. Yijun Ruan
Professor
Genome Institute of Singapore
鈥淐hromatin Looping Architecture and Models of Transcription Regulation in Human Cancer Cells鈥�
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Genomes are known to be organized into 3D structures in vivo through interactions with protein factors for nuclear process such as transcription, and DNA elements separated by long genomic distances are known to functionally interact. However, the details of this view are largely unknown. To explore in this direction, we developed the Chromatin Interaction Analysis using Paired-End-Tag sequencing (ChIA-PET) strategy for de novo detection of genome-wide chromatin interactions, and demonstrated this approach through comprehensive mapping of chromatin interactions involved in transcription regulations mediated by estrogen receptor 伪 (ER伪) in a human genome. In order to map all chromatin loops that are important to chromatin architecture and gene transcription regulation, we have applied the ChIA-PET strategy to general protein factors such as RNA polymerase II (RNAPII) involved in all transcription activities as analysis targets in a number of human cells. Our recent results reveal that large numbers of RNAPII genes are organized into operon-like configuration through long-range chromatin interactions for coordinated transcription regulation. Furthermore, through CTCF ChIA-PET analysis we have discovered the primary role of CTCF is to confer genome-wide 3D framework of chromatin loops for nuclear functions such as transcriptional activation and repression.听 Further analyses of chromatin interactome, transcriptome, and histone modification maps will provide deep insights to advance our understanding of human genome biology.
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Mandatory for Biochemistry听Graduate Students