/tropmed/fr/aggregator Centre J.D. MacLean pour la médecine tropicale et géographique - flux agrégés fr https://pubmed.ncbi.nlm.nih.gov/41224774/?utm_source=Other&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1zSVwQViw4htk0ptJE-rdb4g98Hn0Vy51ceCnixBnFkL6ILsbf&fc=20201106102241&ff=20251114031338&v=2.18.0.post22+67771e2 Trypanosoma cruzi possesses hundreds of genes associated with pathogenesis. The extent and organization of this diverse gene repertoire, expression, and role in infection remain unclear. Using accurate long-read sequencing and chromatin conformation capture, we assembled T. cruzi Sylvio X10 strain chromosomes from telomere-to-telomere. The genome provides accurate organization of multigene family genes, confirming their distribution in expanded clusters or scattered throughout the chromosomes.... Wed, 12 Nov 2025 06:00:00 -0500 https://pubmed.ncbi.nlm.nih.gov/41209419/?utm_source=Other&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1zSVwQViw4htk0ptJE-rdb4g98Hn0Vy51ceCnixBnFkL6ILsbf&fc=20201106102241&ff=20251111002412&v=2.18.0.post22+67771e2 Host specificity is a critical feature of the survival and proliferation of parasites. In the context of interactions with the host, numerous mechanisms have been identified, particularly in parasitic helminths, that enable manipulation of the host immune system to enhance their own survival. The evolutionary history of these interactions often results in hosts becoming disease-tolerant or asymptomatic, even when burdened with a high number of worms, until a disruption in the host's immune... Mon, 10 Nov 2025 06:00:00 -0500 https://pubmed.ncbi.nlm.nih.gov/41182621/?utm_source=Other&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1zSVwQViw4htk0ptJE-rdb4g98Hn0Vy51ceCnixBnFkL6ILsbf&fc=20201106102241&ff=20251104002516&v=2.18.0.post22+67771e2 Understanding and identifying protein-ligand interactions is essential for elucidating fundamental biological processes and developing translational applications such as vaccine and drug targets. Yeast surface display (YSD) systems expressing genome-wide or combinatorial libraries in Saccharomyces cerevisiae are powerful tools to identify protein-ligand interactions in an unbiased fashion. In the following protocol, we couple the YSD system expressing a genome-wide library of the protozoan... Mon, 03 Nov 2025 06:00:00 -0500 https://pubmed.ncbi.nlm.nih.gov/41156739/?utm_source=Other&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1zSVwQViw4htk0ptJE-rdb4g98Hn0Vy51ceCnixBnFkL6ILsbf&fc=20201106102241&ff=20251030004755&v=2.18.0.post22+67771e2 Leishmaniasis is a vector-borne parasitic disease caused by Leishmania spp., transmitted to humans by phlebotomine sand flies. The development of Leishmania into infective metacyclic promastigotes occurs within the sand fly gut, where the bacterial microbiota plays a pivotal role in parasite development and transmission dynamics. This study aimed to characterize the gut bacterial composition of phlebotomine sand flies collected from both endemic (Lalla Aaziza) and non-endemic (Marrakech) regions... Wed, 29 Oct 2025 06:00:00 -0400 https://pubmed.ncbi.nlm.nih.gov/41156623/?utm_source=Other&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1zSVwQViw4htk0ptJE-rdb4g98Hn0Vy51ceCnixBnFkL6ILsbf&fc=20201106102241&ff=20251030004755&v=2.18.0.post22+67771e2 Phlebotomine-borne diseases, transmitted by sand flies, cause significant public health burdens worldwide. In Morocco, Phlebotomus papatasi is a primary vector for Leishmania major and phleboviruses. Despite extensive research in other countries, entomopathogenic parasite investigations in P. papatasi have not been conducted in Morocco until now. This study performed proteomic analysis of female P. papatasi collected from four Moroccan localities using liquid chromatography-tandem mass... Wed, 29 Oct 2025 06:00:00 -0400 https://pubmed.ncbi.nlm.nih.gov/41144209/?utm_source=Other&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1zSVwQViw4htk0ptJE-rdb4g98Hn0Vy51ceCnixBnFkL6ILsbf&fc=20201106102241&ff=20251028002144&v=2.18.0.post22+67771e2 Cryptosporidiosis threatens the lives of young children in developing countries and newborn calves around the world. No vaccine or therapy can prevent or cure this diarrhea-inducing enteric disease caused by Cryptosporidium spp. protozoan parasites. There is an essential need to discover new therapeutic drugs efficient in reducing parasite burden in infected individuals. Research therefore relies on reliable small animal models of cryptosporidiosis. Here, we present excellent mouse models which... Mon, 27 Oct 2025 06:00:00 -0400 https://pubmed.ncbi.nlm.nih.gov/41081009/?utm_source=Other&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1zSVwQViw4htk0ptJE-rdb4g98Hn0Vy51ceCnixBnFkL6ILsbf&fc=20201106102241&ff=20251014005307&v=2.18.0.post9+e462414 CONCLUSIONS: The multifactorial nature of linear growth faltering in these preschool children differed by season. The negative impact of diarrhea emerged only in the rainy season, but the negative impact of sugar intake and the positive impact of fat intake emerged in both seasons. Mon, 13 Oct 2025 06:00:00 -0400 https://pubmed.ncbi.nlm.nih.gov/41072706/?utm_source=Other&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1zSVwQViw4htk0ptJE-rdb4g98Hn0Vy51ceCnixBnFkL6ILsbf&fc=20201106102241&ff=20251011011238&v=2.18.0.post9+e462414 Leishmania donovani is the causative agent of visceral leishmaniasis, a tropical disease affecting millions worldwide. While proteomic studies of Leishmania species have been conducted, the organization of protein-protein interaction (PPI) networks in L. donovani remains largely unexplored. Here, we present a protein interaction network for L. donovani generated through size-exclusion chromatography coupled with mass spectrometry (SEC-MS) and computational analysis. We quantified 3,468 proteins... Fri, 10 Oct 2025 06:00:00 -0400 https://pubmed.ncbi.nlm.nih.gov/41052060/?utm_source=Other&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1zSVwQViw4htk0ptJE-rdb4g98Hn0Vy51ceCnixBnFkL6ILsbf&fc=20201106102241&ff=20251007003115&v=2.18.0.post9+e462414 CONCLUSIONS: This study demonstrates the real-world impact of SVR on liver-related mortality and highlights the value of early treatment and continued support for populations who are marginalised. Mon, 06 Oct 2025 06:00:00 -0400 https://pubmed.ncbi.nlm.nih.gov/41030586/?utm_source=Other&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1zSVwQViw4htk0ptJE-rdb4g98Hn0Vy51ceCnixBnFkL6ILsbf&fc=20201106102241&ff=20251002001916&v=2.18.0.post9+e462414 Background: Primary pulmonary cystic echinococcosis (CE) is a zoonotic disease often caused by Echinococcus granulosus sensu lato complex. Although rare in North America, it can present significant diagnostic and therapeutic challenges. Case Presentation: We report a 36-year-old male from Quebec, Canada, with locally acquired primary pulmonary CE who presented to the emergency department with a two-month history of shortness of breath, cough, and hemoptysis. Laboratory investigations showed mild... Wed, 01 Oct 2025 06:00:00 -0400