DeltaVision OMX Super-Resolution Microscope
Microscope: Applied Precision V4 Blaze OMX system with TIRF option, two Evolve EM-CCD cameras, live cell chamber, DIC optics, in a light-proof enclosure with HEPA-filter, acquisition and analysis workstations
Objectives (only one can be installed at a time):
|
Objective |
|
100x/1.4 oil Olympus, API Certified U-PLANAPO |
|
100x/1.49 TIRF oil Olympus, API Certified U-PLANAPO |
听
Structured Illumination (SIM) and localization microscopy (DLM) modes:
|
Lasers (all diode) |
Emission filters |
Typical fluorophore |
|
405nm, 100mW |
BP 436/31 (420-451nm) |
DAPI |
|
488nm, 200mW |
BP 528/48 (504-552nm) |
GFP, AF488 |
|
568nm, 100mW |
BP 609/37 (591-619nm) |
mCherry, AF568 |
|
642nm, 300mW |
BP 683/40 (663-703nm) |
AF647 |
听
听Conventional wide-field fluorescence:
Light source: 6-color InsightSSI (solid state illumination) module
Fluorescence filters:
|
Excitation Wavelengths |
Emission filters |
Typical fluorophore |
|
381-399nm |
BP 436/31 (420-451nm) |
BFP, DAPI |
|
426-450nm |
BP 478/35 (460-495nm) |
CFP |
|
461-489nm |
BP 528/48 (504-552nm) |
GFP, FITC, Alexa 488 |
|
505-515nm |
BP 541/22 (530-552nm) |
YFP, Alexa 532 |
|
563-588nm |
BP 609/37 (590-618nm) |
mCherry, Alexa 568 |
|
621-643nm |
BP 683/40 (663-703nm) |
Cy5, Alexa 647, 635, 660 |