Spinning Disk (SD3) Confocal
Microscope: Quorum WaveFX-X1 spinning disk confocal system, on a Leica DMI6000B inverted microscope, fully motorized, with an ASI MS-2000 piezo stage, and two Photometrics PRIME BSI CMOS cameras; Live Cell Instruments Chamlide TC environmental control system
Equipped for photo-stimulation (laser ablation, FRAP/FLIP, optogenetics): Micropoint Target Illumination System and Mosaic Digital Diaphragm System
Allows precise, targeted photo-manipulation in combination with simultaneous dual-color imaging at high resolution and speed
Objectives:
|
Position |
Objective |
Phase/DIC |
|
1 |
10x/0.4 dry HC PL APO; 506284 |
听 |
|
2 |
20x/0.7 dry HC PLAN APO; 506166 |
DIC |
|
3 |
40x/0.85 dry corr 0.11-0.23 HCX PL APO; 506294 |
DIC |
|
4 |
40x/1.25-0.75** oil lBL HCX PL APO; 506253 |
DIC |
|
5 |
63x/1.40-0.60** oil lBL HCX PL APO; 506192 |
DIC |
|
6 |
Micropoint alignment device |
听 |
** must be adjusted for highest NA
听
Confocal mode: Lasers, fluorescence emission filters:
|
Lasers (all diode) |
Emission filters |
Emission filters |
Typical fluorophore |
|
446nm, 40mW |
HQ 470/40 |
HQ 470/40 |
CFP |
|
491nm, 50mW |
ET 525/50 |
ET 525/50 |
GFP |
|
ET 540/40 |
听 |
YFP |
|
|
568nm, 50mW |
FF 593/40 |
FF 593/40 |
OFP (orange) |
|
ET 620/60 |
听 |
RFP/mCherry |
|
|
643nm, 110mW |
ET 700/75 |
听 |
FarRedFP |
Additional filters for main camera (wheel 2, orange and red emitters): ET 620/60, HQ 650/100, ET 700/75, HQ 600 LP, HQ 655 LP
听
Wide-field fluorescence:
Light source: X-Cite120Q metal-halide fluorescence lamp, 120W
Fluorescence filter cubes, Leica:
|
Filter set |
Excitation filter |
Dichroic mirror |
Emission filter |
Typical fluorophore |
|
CGFP |
BP 436/20 |
455 |
BP 480/40 |
CFP |
|
GFP |
BP 470/40 |
500 |
BP 525/50 |
GFP |
|
TX2 |
BP 560/40 |
595 |
BP 645/75 |
Texas Red |